Abstract
The author obtained a conjugate of botulinus rabbit antiserum with fluorescein isocyanate, possessing immunological specificity. In staining the films of pure cultures ofCl. botulinum by the luminescent antiserum, an intensive green luminescence of these microorganisms was obtained. When preparations of other anaerobic cultures,E. coli, staphylococci or anthracoids were stained with the same conjugate, the specific luminescence was absent. In this way it is possible to reveal solitaryCl.botulinum cells in the films prepared from suspensions with a concentration of up to 500,000 bacterine cells per 1 ml. The method of staining by luminescent antibodies makes it possible to identifyCl. botulinum in microscopic preparations.
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