Abstract
Summary A thin-layer chromatographic method is described for the separation of atropine from its degradation products on silica gel HF2s4, with acetone-7.5%v/v ammonium hydroxide (9:1) as the solvent for development. Atropic acid and tropic acid are detected as magenta spots by examination under screened ultraviolet radiation at 254 nm. After the plate has been sprayed with potassium iodobismuthate solution, tropine is detected as a deep purple spot whereas atropine and apoatropine form orange spots on a very pale orange background. The method is simple, rapid and of potential value in quality-assurance laboratories of hospital pharmacies as a limit test for degradation products of atropine.
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