Abstract
The APOE ϵ4 gene is a susceptibility gene or risk factor for Alzheimer's disease. The gene codes for a protein which limits the brain's ability to break down amyloid-beta, a protein that is toxic to the brain. In this study, we have prepared magnetic beads - CdSe/ZnS QDs-APOE probe DNA complexes for the detection of APOE DNA. QDs were conjugated to amine- functionalized magnetic beads by EDC/Sulfo-NHS coupling reaction. Thiol group modified single stranded probe oligonucleotides of APOE ϵ2 and APOE ϵ4 were immobilized on the surface of QDs to prepare magnetic bead-QDs-DNA complexes. APOE genes could be detected by fluorescence quenching of QDs by intercalation dye after hybridization. PDMS based micro-fluidic chips were prepared and the bead-QDs-DNA complexes were packed on the channel of the microfluidic chip. The APOE ϵ2 and APOE ϵ4 gene could be detected via fluorescence quenching of QDs on the channel.
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