Abstract
Three modified nucleosides were designed with the aim of achieving triplet formation with the CG base pair of duplex DNA. Direct anthraniloylation of 2′-deoxycytidine, using isatoic anhydride, afforded the novel N 4-anthraniloyl-2′-deoxycytidine. Much improved preparations of N 4-carbamoyl-2′-deoxycytidine and of N 4-ureidocarbonyl-2′-deoxycytidine were accomplished. The modified nucleosides were incorporated into oligonucleotides. Thermal denaturation studies and gel mobility shift analysis suggest that these nucleosides do not form base triplets with any of the four base pairs of DNA.
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have