Abstract
Thermalcycler was extensively used machine for amplify DNA sample. One of the major problems in the working time was that it spent most of time for cooling and heating. In order to improve the efficient, the study presented a novel method for amplify DNA sample. For this concept, the DNA sample in the silicon chamber which was pushed by a tappet through the three temperature regions around a center and then the DNA segments could be amplified rapidly after 30 cycles. The polymerase chain reaction (PCR) platform was composed of the thin-film heaters, Cu plates, DC powers, and temperature controllers. The photolithography and bulk etching technologies were utilized to construct the thin-film heater and DNA reaction chambers. Finally, 1 μl 100 bp DNA segment of Escherichia coli K12 was amplified successfully within 36 min on this PCR platform.
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