Abstract
Metarhizium anisopliae is a parasitic fungus on insects, and thus called entomopathogenic fungus. This fungus is used as a biological control agent for insect pests. Fungal propagation can be done using a variety of media. The purpose of this study was to analyze the growth of M. anisopliae on four types of media, with conidial density and viability as the growth parameters. This research was conducted at the Laboratory of Microbiology, Universitas Negeri Semarang. This study was an experimental research used a Completely Randomized Design (CRD) with one factor and four treatments: Control (PDA/Potato Dextrose Agar medium), Treatment I (ELSA/Extract Larvae Sucrose Agar medium), Treatment II (CWSA/Coconut Water Sucrose Agar medium), Treatment III (CWELSA/Coconut Water and Extract Larvae Sucrose Agar medium). The and results showed that there was an effect of growth media on the density and viability of M. anisopliae conidia. CWELSA media had the highest conidial density (2.91 x 108 cfu/mL) and viability (97.17%). CWSA media had 2.82 x 108 cfu/mL and 95.33%. PDA media had 2.25 x 108 cfu/mL and 92.83%. ELSA media had 1.64 x 108 cfu/mL and 90.83%. The high conidial density and viability of M. anisopliae is CWELSA medium. This study is as an alternative growth medium to improve the quality of M. anisopliae propagation.
Highlights
Entomopathogenic fungi have potential as biological agents for controlling pest infestation (Rai et al, 2014; Indriyanti et al, 2018a)
The results of the One Way ANOVA analysis at the 5% level obtained the Sig. value of 0.00 < 0.050, so that there was an influence of growth media on the number of M. anisopliae conidial density
Tukey's HSD analysis showed that CWELSA and CWSA medium had the highest effectiveness compared to ELSA and Potato Dextrose Agar (PDA) on the conidial density of M. anisopliae
Summary
Entomopathogenic fungi have potential as biological agents for controlling pest infestation (Rai et al, 2014; Indriyanti et al, 2018a). One of the entomopathogenic fungal species that can be used as biological controlling agent is Metarhizium anisopliae (Indriyanti et al, 2017). This entomopathogenic fungus has been known to be very effective in controlling several pest species. It has a wide range of insect hosts including Lepidotera, Coleoptera, Hemiptera, Diptera, and Hymenoptera (Moonjely & Bidochka, 2019; Lovett et al, 2015). In addition to grow microorganisms, the medium can be used for isolation and propagation as well as for testing their physiological properties and calculating the the number of microorganism (Nuryanti et al, 2012)
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