7-days of FREE Audio papers, translation & more with Prime
7-days of FREE Prime access
7-days of FREE Audio papers, translation & more with Prime
7-days of FREE Prime access
https://doi.org/10.1016/s0378-5955(02)00513-0
Copy DOIJournal: Hearing Research | Publication Date: Aug 14, 2002 |
Citations: 18 |
The objectives of the current study were to investigate the distribution and production of NO in cochlear blood vessels, and to assess whether the inhibition of basal NO production leads to vascular protein leakage of the cochlear microvasculature. Using the fluorescent dye 4,5-diaminofluorescein diacetate, NO was detected, both in vitro and in vivo, in the endothelial cells of various cochlear blood vessels, including the spiral modiolar artery, the vessel of the basilar membrane and the vessels of the spiral osseous lamina. Vessel leakage was assessed using intravital fluorescence microscopy following systemic infusion of fluorescein isothiocyanate-labeled bovine albumin. Local perfusion of the cochlear basal turn with either Ringer’s solution or Ringer’s containing an inactive nitric oxide synthase inhibitor (100 μM) produced minimal protein leakage. Perfusion with the nitric oxide synthase inhibitor N G-nitro- L-arginine methyl ester (100 μM) produced significantly enhanced vascular protein leakage. The findings demonstrate the presence of endothelial NO in the cochlear blood vessels and suggest that NO protects cochlear venules against excessive vascular leakage.
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Disclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.