The deleterious effect of diatoms on the biomass and growth of early stages of their copepod grazers

Abstract

Diatoms are key phytoplankton species in the world oceans and essential in the transfer of energy through marine food chains. Their beneficial role has been questioned after the discovery that some diatom species produce teratogenic compounds such as polyunsaturated aldehydes and other oxylipins that induce abortions, poor larval development and high offspring mortality in many invertebrates. To test whether maternal and post-embryonic diatom diets affected development in the copepod Paracartia latisetosa, we carried out experiments in which the PUA-producing diatom Skeletonema marinoi (SKE) was fed to this copepod species. Controls were run with the dinoflagellate, Prorocentrum minimum (PRO), which does not produce any of these compounds. Four treatments were tested: both mothers and progeny always fed on either SKE or PRO, and those in which mothers received either PRO or SKE, and neonates were switched to the other diet. Our results show that a SKE/SKE diet induced lower egg production and egg viability, as well as slower embryonic development than the control diet. Naupliar development was negatively affected with the diatom diet which blocked the metamorphosis of larvae at N3, 4days after hatching, compared to the control diet that allowed for normal naupliar development in 10days. Nauplii reared on PRO and spawned by females fed on SKE were able to complete development, but only after 11days, whereas larvae reared on SKE spawned by females fed on PRO were arrested at the N5 developmental stage, 12days after hatching. Apoptotic TUNEL-positive nauplii were detected with the SKE diet, indicating imminent death. Significant differences were also recorded by comparing biomass of equivalent naupliar stages obtained for each treatment, and the overall mean biomass female−1day−1 for each diet. Growth inhibition due to oxylipin-producing diatoms may have important consequences on prey–predator relationships and on the biomass flux through marine food chains.