Abstract
THE DEAMINATION OF CYTIDINE IN ACID SOLUTION AND THE PREPARATION OF URIDINE AND CYTIDINE BY ACID HYDROLYSIS OF YEAST NUCLEIC ACID
Highlights
BY HUBERTUnder extremely vigorous conditions (2 hours at 175” in 25 per cent sulfuric acid) the free pyrimidines, uracil and cytosine, have been the products isolated (3)
Stability of Pyrimidine Nucleosides As Compared to Rates of Liberation of Inorganic Phosphate from Cytidylic Acid and from Yeast Nucleic AcidThe stability of cytidine to acid hydrolysis was determined by refluxing solutions containing 0.4 mg. of cytidine per ml. in 0.1, 0.2, 1, and 5.5 N sulfuric acid and in 1 and 6 N hydrochloric acid for 24 and 48 hours
The stability of cytidine and uridine to acid hydrolysis has been determined in comparison with the rates of liberation of inorganic phosphate from cytidylic acid and from yeast nucleic acid
Summary
Under extremely vigorous conditions (2 hours at 175” in 25 per cent sulfuric acid) the free pyrimidines, uracil and cytosine, have been the products isolated (3). That it is possible to prepare cytidine after hydrolysis of cytidylic acid or nucleic acid with 10 per cent sulfuric acid at 130” in a sealed tube was shown by Levene (4) and by Loring and Pierce (5) in experiments in which either cytidine was the only product isolated or the yield of cytosine was relatively small. The second procedure requires a relatively expensive reagent and in our laboratory has given low yields of cytidine (5).r In an attempt to improve the method for the preparation of these compounds, experiments were conducted to determine the usefulness of acid hydrolysis under reflux conditions as a method of obtaining cytidine and uridine.
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