Abstract
During the last 10 years an increasing number of plants for re-use of refuse have been constructed in Europe and the U.S.A. During the same period several cases of occupational respiratory diseases among workers have been reported in the recycling industry. The aim of this project was to show,in vitro, if there is any change in the cytotoxic potential of garbage dust during the process of converting household waste to compost. Two cell lines have been exposed to extracts of waste fuel pellets and compost, taken from three different time periods in the composting process. Significant differences were found in the cytotoxic potential of extracts of household waste (P<0.05). Extracts of 0.48 mg well−1raw compost, fresh compost and matured compost show a cytotoxic effect at 97, 41 and 44%, respectively of unexposed cells. In conclusion, these results show the greatest cytotoxic potential when the microbial activity seems to be at its height in the composting process. Earlier, studies on the effect of endotoxin (lipopolysaccharide, LPS) on the cells, and with pure endotoxin did not find any cytotoxic effect in the assay. Further investigations are needed in order to find which micro-organisms or components from these are responsible for the cytotoxic potential.
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