Abstract
A concept of using low intensity light therapy (LILT) as an alternative approach to cancer treatment is at early stages of development; while the therapeutic effects of LILT as a non-invasive treatment modality for localized joint and soft tissue wound healing are widely corroborated. The LEDs-based exposure system was designed and constructed to irradiate the selected cancer and normal cells and evaluate the biological effects induced by light exposures in visible and infrared light range. In this study, human breast cancer (MCF7) cells and human epidermal melanocytes (HEM) cells (control) were exposed to selected far infrared light (3400nm, 3600nm, 3800nm, 3900nm, 4100nm and 4300nm) and visible and near infrared wavelengths (466nm, 585nm, 626nm, 810nm, 850nm and 950nm). The optical intensities of LEDs used for exposures were in the range of 15µW to 30µW. Cellular morphological changes of exposed and sham-exposed cells were evaluated using light microscopy. The cytotoxic effects of these low intensity light exposures on human cancer and normal cell lines were quantitatively determined by Lactate dehydrogenase (LDH) cytotoxic activity and PrestoBlueTM cell viability assays. Findings reveal that far-infrared exposures were able to reduce cell viability of MCF7 cells as measured by increased LDH release activity and PrestoBlueTM assays. Further investigation of the effects of light irradiation on different types of cancer cells, study of possible signaling pathways affected by electromagnetic radiation (EMR) and in vivo experimentation are required in order to draw a firm conclusion about the efficacy of low intensity light as an alternative non-invasive cancer treatment.
Highlights
Conventional methods such as chemotherapy, radiotherapy, and therapeutic purposes, there is a growing need to adopt reverse surgery have shown a limited success and effectiveness in treatment of engineering methods, i.e. activation of a certain biological activity that melanoma
Further investigation of the effects of light irradiation on different types of cancer cells, study of possible signaling pathways affected by electromagnetic radiation (EMR) and in vivo experimentation are required in order to draw a firm conclusion about the efficacy of low intensity light as an alternative non-invasive cancer treatment
When comparing the images obtained from the exposed well to the unexposed specimen, it can be inferred that irradiation with wavelength of 3400nm, 3600nm, 3800nm, 3900nm, 4100nm and 4300nm induced detrimental effects on MCF7 cells
Summary
Conventional methods such as chemotherapy, radiotherapy, and therapeutic purposes, there is a growing need to adopt reverse surgery have shown a limited success and effectiveness in treatment of engineering methods, i.e. activation of a certain biological activity that melanoma. The primary reason for for medical applications [2] To this end, there have been several cancer development is permutations in the DNA of a cell that lead to attempts in recent decade to induce biological function through uncontrolled growth of mutated cells that contribute to tumor irradiation of cells/molecules by an external source of light [3-5]. Oncogenes are a specific group of growth effectors that These induced biological functions are the result of energy transferred promote uncontrolled cell growth and proliferation. These proteins to the living organism [6-8]. The changes in quantum-mechanical are derived from normal cellular growth effectors (so-called proto- energy states of photosensitive molecules would result in the oncogenes) by a limited number of modifications: mutations, frequency selectivity of many light induced biological processes [9-
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