The Cytotoxic Effects of Humic Acid on Human Breast Cancer Cells

Asli Aykac,Meryem Güvenir,Kaya Süer,Tuğçe Balcı Okcanoğlu,Eda Becer,Seda Vatansever

doi:10.3390/proceedings2251565

Asli Aykac, Meryem Güvenir + Show 4 more

Open Access

https://doi.org/10.3390/proceedings2251565

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Publication Date: Dec 7, 2018
Citations: 3	License type: CC BY 4.0

Affiliation: Near East University, Manisa Celal Bayar University

Abstract

Breast cancer is the most common cancer among women and in order to create alternative treatments different types of in vivo and in vitro studies have used various plant-based therapeutic agents. Humic acid (HA) induces apoptosis and has various pharmacological properties including anti-inflammatory and anti-proliferative effects. In our study, we examined the cytotoxic effects of HA at concentrations of 5, 10, 20, 50 and 100 μg/mL in human breast adenocarcinoma MCF-7 cell line for 24 and 48 h. By using MTT method, it has been found out that HA 100 g/mL had cytotoxic effect on human breast adenocarcinoma MCF-7 cell line at both 24 and 48 h; also found out that the effective dose of HA at the same time (24 and 48 h) was 50 μg/mL. The results of our study will shed light on the development of alternative therapeutic approaches in the treatment of cancer by evaluating the cytotoxic effect of HA.

Highlights

In recent years, the use of natural products and medicinal plants has become increasingly widespread in order to protect the general health of individuals, to prevent and treat diseases.Nowadays, interest in antioxidant compounds that can delay or prevent oxidative damage by inactivating free radicals is increasing worldwide
Human breast adenocarcinoma MCF-7 cells were treated with Humic acid (HA) at concentrations of 5, 10, 20, 50 and 100 μg/mL for 24 and 48 h
Our results show that HA prepared at 100 μg/mL dose had a cytotoxic effect at 24 and 48 h (p < 0.005) while the 50 μg/mL HA was determined to be the most effective dose on MCF-7 cell lines at 24 and 48 h (Figure 2)

Summary

Introduction

The use of natural products and medicinal plants has become increasingly widespread in order to protect the general health of individuals, to prevent and treat diseases. Humic acid (HA), is the most active ingredient of the main content of organic substances in the soil called humus. Reactive groups such as carboxyl, phenol, quinone in HA molecules give mineral and antioxidant properties to acids [1]. Many researchers have suggested that HA has the power to protect against cancers and cancer-causing viruses. This suggests that HA has different biological activities such as cancer prophylaxis and treatment, as well as decreased cell proliferation and decreased angiogenesis [7]. The cytotoxic effect of HA in human breast adenocarcinoma MCF-7 cell line was aimed in this study by measuring cell viability with MTT assay

Purification of Humic Acid

Cell Line and Cell Culture

Results and Discussion

Conclusions