Abstract
Potent, structurally different tumor promoters inhibited growth of 6 human mammary carcinoma cell lines (ROOS et al, PNAS in press). This growth inhibition was investigated by measuring the phorboid receptor binding using [ 3H] PDBu (4β-phorbol 12,13 dibutyrate). Specific, high affinity receptors were found in all six cell lines. [ 3H] PDBu binding affinities were higher in the cytosolic fractions than in the corresponding intact cells (K α = app. 1nM vs K α = app. 15nM). The hormone-independent cell lines (BT-20, HBL-100 and MDA-MB-231) exhibited significantly higher levels of cytosolic [ 3H] PDBu receptors than the hormone-dependent cells (MCF-7, T-47-D and ZR-75-1). The subcellular distribution of the [ 3H] PDBu binding correlated well with the distribution of the protein kinase C activity (r = 0.95).
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