THE CYTOPROTECTIVE ACTIVITY OF AQUEOUS GREEN TEA EXTRACT AGAINST METRONIDAZOLE AND TINIDAZOLE GENOTOXIC EFFECT

Abstract

Objective: The study was designed to evaluate the genoprotective effect of green tea extract against genotoxicity induced by metronidazole and tinidazole.Methods: A total of 36 mice were used, for each experiment. The animals were allocated into six groups: Group I - negative control administered distilled water; Group II - healthy mice treated with metronidazole alone, Group III - healthy mice treated with tinidazole alone, Group IV - healthy mice administered green tea extract alone, Group V - healthy mice administered of metronidazole anddd then green tea extract was administered, and Group VI - healthy mice administered of tinidazole and then green tea extract was administered.Results: It has been found that there are significant differences in mitotic index and micronucleus (MN) appearance between the Groups II, IV, and V (7.76±0.8, 11.92±1.14, and 8.36±0.57 and 6.92±0.5, 10.18±1.19, and 7.52±1.05) in bone marrow cells and spleen cells for mitotic index, respectively (6.75±0.4, 5.5±0.41, and 5.92±0.68), in bone marrow for MN appearance. Furthermore, it has been found that there are significant differences in mitotic index and MN appearance between the Groups III, IV, and VI (4.36±0.88, 11.92±1.14, and 5.36±0.55 and 4.08±0.35, 10.18±1.19, and 5.08±0.35) in bone marrow cells and spleen cells for mitotic index, respectively (8.43±0.96, 5.5±0.41, and 6.84±0.66), in bone marrow for MN appearance.Conclusion: The aqueous extract of green tea has a protective effect against the genotoxic effects induced by either metronidazole or tinidazole in mice.