Abstract
Abstract. Tick-borne encephalitis (TBE) is severe neuroinfectious disease with involvement of immune mechanisms in pathogenesis. Comparative analysis of synthesis of key cytokines had been performed for the TBE virus (TBEV) infected cells and in the presence of inactivated vaccine against TBE in vitro. Persistent TBEV infection of immortal tissue culture of human larynx cancer cells caused transcription activation of interferons IFNα, IFNγ, IFNλ1, interleukins IL-1β, IL-2, IL-4, IL-8, IL-10, IL-12, tumour necrosis factor TNFα as well as one of apoptosis factors Fas. Comparison of transcription and production of cytokines revealed that the TBEV infection resulted in posttranscription Th1 shift of cytokine response. In the presence of inactivated vaccine against TBE based on the same strain Sofjin of the TBEV activation of transcription of cytokines IFNα, IFNλ1, IL-4, IL-10 was also observed as after the TBEV infection that together with an additional stimulation of GM-CSF production might serve as an evidence of Th2 response. Involvement of IFNIII type (IFNλ1) both during persistent infection and after addition of inactivated vaccines was found in the first time. Differences in dynamics of cytokines IL-2, IL-8, IL-10, IL-12, TNFα response during the TBEV infection and in the presence of inactivated vaccine are described.
Highlights
Comparative analysis of synthesis of key cytokines had been performed for the Tick-borne encephalitis (TBE) virus (TBEV) infected cells and in the presence of inactivated vaccine against TBE in vitro
Comparison of transcription and production of cytokines revealed that the TBEV infection resulted in posttranscription Th1 shift of cytokine response
In the presence of inactivated vaccine against TBE based on the same strain Sofjin of the TBEV activation of transcription of cytokines IFNα, IFNλ1, IL-4, IL-10 was observed as after the TBEV infection that together with an additional stimulation of GM-CSF production might serve as an evidence of Th2 response
Summary
Проведен сравнительный анализ синтеза ключевых цитокинов при инфекции ВКЭ и в присутствии инактивированной вакцины против КЭ in vitro. В присутствии инактивированной вакцины против КЭ, основанной на том же штамме Софьин ВКЭ, также происходила активация транскрипции цитокинов IFNα, IFNλ1, IL-4, IL-10, как и в результате инфекции штаммом Софьин ВКЭ, а также продукция GM-CSF, что свидетельствует об индукции преимущественно Th2 пути. Активность цитокинов (IL-1β, IL-2, IL-4, IL-6, IL-10, IL-12, TNFα и IFNγ) после иммунизации мышей инактивированной вакциной против КЭ свидетельствует об активации макрофагов, Ти В-лимфоцитов [8], что не типично для очищенных белков, являющихся экзогенными антигенами и поэтому индуцирующих иммунный ответ по Th2 пути с секрецией IL-4 и IL-5, но не IFNγ [7]. Ивановского МЗ РФ, Москва; Антошина И.Ф., соискатель лаборатории культур тканей ФГБУ НИИ вирусологии им. Ивановского МЗ РФ, Москва; Морозова О.В., д.б.н., ведущий научный сотрудник лаборатории иммунологии ФГБУ НИИ вирусологии им.
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