Abstract
Human peripheral leukocyte cultures were exposed to ozone at 12 and 36 hours after stimulation with phytohaemagglutinin (PHA). Two methods were used. (1) Ozone was bubbled through a suspension of cells in Hanks' Balanced Salt Solution, or (2) cells were exposed to ozone-saturated phosphate-buffered Saline D solutions. In all, 23 different combinations of dose and time of exposure were used. Although there was no consistent effect observed on the induction of chromosome-type aberrations in the 12-hour poststimulation cultures, there did appear to be a slight, but significant, increase in the frequency of chromatid-type aberrations observed in the cultures exposed to 7.23 and 7.95 ppm/hour of bubbled ozone 36 hours after stimulation. The significance of this result is discussed. Young adult male mice were also given various ozone exposures in an inhalation chamber. Short-term cultures were made of their peripheral leukocytes from immediately to 2 weeks after exposure; there was no significant level of chromosome damage at any of the sampling times. Analysis of primary spermatocytes from these same males 8 weeks after exposure yielded no recognizable reciprocal translocations.
Published Version
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