Abstract
The root cap of corn (Zea mays) is comprised of several different types of cells, each having a unique structure and function. For example, columella cells in the center of the cap are responsible for perceiving gravity. These cells subsequently differentiate into peripheral cells, which are located at the edge of the cap and function in the production and/or secretion of mucopolysaccharides (mucilage). Differences in enzymatic activity and location in cells of the root cap provide key evidence for the nature and site of the functional changes that accompany differentiation.The cytochemical localizations of glucose-6-phosphatase (G-6-Ptase) and catalase were undertaken to define the modifications of enzyme localization associated with cellular differentiation in root caps of Zea mays. G-6-Ptase and catalase were localized using procedures described by Hall. Cytochemistry as performed in this study was consistently specific for the designated enzyme and gave precise ultrastructural localization. Enzymatic precipitate was absent in all controls. Sections were not counterstained to allow for positive identification of staining due to enzymatic activity.
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Schedule a callMore From: Proceedings, annual meeting, Electron Microscopy Society of America
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