The Cytoarchitecture of the Telencephalon of Betta Splendens Regan 1910 (Perciformes: Anabantoidei) with a Stereological Approach to the Supracommissural and Postcommissural Nuclei.

Abstract

Teleostean fish brains are useful models to study cellular and functional specializations along the phylogenesis. The Betta splendens Regan 1910 (Siamese fighting fish; Perciformes:Anabantoidei) is known for its aggressive display, courtship behavior, nest building, and offspring care. Here, we present novel and detailed data about the cytoarchitecture of the olfactory bulb and the telencephalic hemispheres of this fish. The hematoxylin-eosin and Nissl techniques served to identify brain nuclei (n = 19 males and n = 21 females) and for the stereological evaluation of the numerical density of cells and the proportion of neurons and glial cells in the ventral telencephalon supracommissural (Vs) and postcommissural (Vp) nuclei of adult males and females. These nuclei are putative homologs of the sexually dimorphic medial amygdala in mammals. The olfactory bulb of Betta splendens consists of 5 concentrically arranged layers plus ganglion cells of the terminal nerves. The dorsal telencephalon consists of 16 different cell groups. The ventral telencephalon has 8 nuclei, plus the lateral septal organ and the nuclei of the preoptic area forming an anatomical continuum. The rostrocaudal extent of the Vs and Vp is not different between sexes. In both nuclei, the proportion of neurons to glial cells is approximately 2:1 and the density of neurons and glial cells is not different between sexes. These morphological findings can subserve future research on the brain function of the Betta splendens and the search for neural sex differences in other central areas of this same species, in other teleost species, or yet in other related vertebrate group. Anat Rec, 00:000-000, 2017. © 2017 Wiley Periodicals, Inc. Anat Rec, 301:88-110, 2018. © 2017 Wiley Periodicals, Inc.