Abstract

A culture method is described by which mature organs, or parts thereof, from rats and mice may be maintained in vitro in a simple synthetic medium for the purpose of short term experiments. The following organs could regularly be kept in a satisfactory state of histological preservation for 6–9 days: ureter, ductus deferens, uterus, trachea, arteries, salivary glands, mammary gland, prostate, seminal vesicle, lung, thyroid, parathyroid, pituitary, pineal, ovary, skin, white adipose tissue, lymph nodes, symphathetic ganglia. Partial survival was obtained in the case of kidney, adrenal and spinal ganglion. The survival of brain, liver, thymus, spleen, bone marrow, testis and pancreas was unsatisfactory.

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