Abstract
Objective To establish a method for creating rabbit tracheal epithelial cell sheets in vitro.Methods Rabbit tracheal epithelial cells were isolated and cultured using tissue explant technique,and passaged in UpCell culture dishes at 37 ℃,5％ CO2 and saturated humidity after being identified by cytokeratin (CK)-18 staining.About two days,cells have reached confluency,and tracheal epithelial cell sheet was prepared when the ambient temperature was changed to 20 ℃.Results Epithelial cells were isolated from rabbit tracheal mucosa using tissue explant technique successfully,and all cells were positive for CK-18 staining,showing they had properties of epithelial cells.When the ambient temperature dropped at 20 ℃,epithelial cells detached automatically from the UpCell culture dishes and formed an intact cell sheet.The cells are strongly attached and overlapping under light microscope.Conclusion Rabbit tracheal epithelial cell sheet can be fabricated with UpCell culture dishes and it could provides a novel pathway for constructing tissue engineering trachea. Key words: Rabbit; Epithelial cell; Cell sheets technology; Tissue engineered trachea; UpCell culture dishes
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