The crayfish as a “biological indicator” of aquatic contamination by heavy metals

Abstract

Cu, Cd. Mg, Mn and Ni concentrations in whole Cambarus bartoni, trapped from Nepahwin Lake, Joe Lake and Wizard Lake, lying at distances of 10, 30 and 150 km, respectively, from the Sudbury smelters in Northeastern Ontario, showed an inverse relationship with the distance of the habitat from the emission site. Highest concentrations of Cu were observed in the hepatopancreas, Cd in the hepatopancreas and the gut, Fe and Mn in the gut, and Mg in the exoskeleton. Tissue concentrations of Ni and Zn were highly variable. Differences between males and females at each sampling site were not significant at the 5% level. The general relationship between crayfish tissue metal concentrations at the three sites was Cu > Mg > Mn > Zn > Ni > Cd. This observed relationship, except for Mg and Mn, agreed with the concentration relationship of these metals in the water and the sediments (top 10–20 cm) in the three lakes. In laboratory studies, Cu concentrations in the hepatopancreas, gills, exoskeleton and the viscera increased significantly over the control during the 4-weeks exposure to 125,250 and 500 μg Cu 1 −1. Hepatopancreas, in general, had the highest Cu concentration, and the exoskeleton, the lowest. Relationship between Ni exposure and the tissue Ni concentrations in the four treatments was 800 μg Ni l −1 > 400 μg Ni l −1 = 200 μg Ni l −1 > control, while between the exposure periods and the tissue Ni concentrations was week 4 > week 1 > week 3 = week 2. Highest Ni concentrations at the end of the 4-week exposure to Ni were observed in the gut, gills and the hepatopancreas, and the lowest in the viscera. The present study suggests that Cambarus bartoni is a reliable indicator of the presence of class B and borderline elements with specific gravity > 5 in the aquatic environment. However, their value as biomonitors of variations in Cu and Ni concentrations in freshwater ecosystems is debatable.