Abstract
The mitochondrial genome encodes key electron transfer chain (ETC) proteins which produce the vast majority of cellular ATP. Sequence analysis of maternal inherited mitochondrial DNA is an effective way to assess the individual characteristics of commercial breeding lines of pigs. The aims of our study were to analyze the nucleotide sequence of the mitochondrial gene COXII in Large White pigs, to search for polymorphisms and to evaluate their associations with pigs productive abilities and the backfat thickness. The studied pigs of Large White were divided in two groups. The first group (LW-1) was consisted of pigs bred in Russian Federation since 1998. The second group (LW-2) included pigs of Large White imported in 2014 to Russia from England. To evaluate the associations of the COXII gene haplotypes with productive qualities, 96 sow LW-1 were selected. The variability analysis of the COXII revealed the presence of three haplotypes: Hap_1 (TTTCTGGAT, in positions m.8292, m.8334, m.8419, m.8466, m.8526, m.8634, m.8664, m.8682, m.8682, m.8694 respectively), Hap_2 (CCCCCAAGC) and Hap_3 (TTTTTGGAT). We have obtained the results on the association of the COXII polymorphic variants in the loci (m.C8292, m.C8334, m.C8419, m.C8526, m.A8634, m.A8664, m.G8682, m.C8694) that are typical for Hap_2, with higher value of backfat thickness in pigs.
Highlights
Mitochondria play an important role in metabolic processes as well as in apoptosis and cell aging
Polymorphism identified in mitochondrial DNA is one of the most common genetic markers used in population studies of many animal species
The aim of this study was the investigation of the mtDNA COXII gene nucleotide sequence in Large White pigs, the identification of polymorphisms and the evaluation of their associations with the productive qualities of pigs
Summary
Mitochondria play an important role in metabolic processes as well as in apoptosis and cell aging. It’s caused by the high rate of mtDNA mutations and the fact of the strict maternal fashion inheritance of mitochondria. Both male and female individuals get mitochondria from the cytoplasm of the maternal egg. MtDNA is not able to recombination, the entire molecule is changed only by mutation for thousands of years (Fernandez et al, 2008; Nisztuk-Pacek et al, 2018). Pigs mtDNA is an annular molecule consisting of average of 16.5 kbp In pigs it includes 37 genes: 13 for proteins of the Electron Transfer Chain (ETC), 22 for tRNA and two rRNAs (16S rRNA and 12S rRNA), as well as the most variable region, the D-loop (Yen et al, 2007)
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