The cotton MAPK kinase GhMPK20 negatively regulates resistance to Fusarium oxysporum by mediating the MKK4–MPK20–WRKY40 cascade

Abstract

Fusarium wilt is one of the most serious diseases affecting cotton. However, the pathogenesis and mechanism by which Fusarium oxysporum overcomes plant defence responses are unclear. Here, a new group D mitogen-activated protein kinase (MAPK) gene, GhMPK20, was identified and functionally analysed in cotton. GhMPK20 expression was significantly induced by F.oxysporum. Virus-induced gene silencing (VIGS) of GhMPK20 in cotton increased the tolerance to F.oxysporum, whereas ectopic GhMPK20 overexpression in Nicotiana benthamiana reduced F.oxysporum resistance via disruption of the salicylic acid (SA)-mediated defence pathway. More importantly, an F.oxysporum-induced MAPK cascade pathway composed of GhMKK4, GhMPK20 and GhWRKY40 was identified. VIGS of GhMKK4 and GhWRKY40 also enhanced F.oxysporum resistance in cotton, and the function of GhMKK4-GhMPK20 was shown to be essential for F.oxysporum-induced GhWRKY40 expression. Together, our results indicate that the GhMKK4-GhMPK20-GhWRKY40 cascade in cotton plays an important role in the pathogenesis of F.oxysporum. This research broadens our knowledge of the negative role of the MAPK cascade in disease resistance in cotton and provides an important scientific basis for the formulation of Fusarium wilt prevention strategies.