The Correlations between Capacity Factors k′ and RM Values in Liquid Column and Thin-Layer Chromatography

Abstract

Abstract The purpose of this studie being carried out is to define some regularities between Thin-layer and Liquid Column Chromatography; i.e. between experimental obtained and theotetical calculated RM-values and experimental obtained logk-values for some non-active organic substances in mixed binary solvents systems. It was studied the kind these correlations change with changing mobile phase compositions. The present paper is a certain aspect of new studies on possibilities of the use Thin-layer Chromato-graphy as a pilot technique for Liquid Column Chromatography (1,2). In a study on the optimization of chromatographic process it is necessary to define the correlations between RM-values which characterize the retention in TLC and k′-values which characterize the retention in in LCC. In the paper the changes of these correlations with the changes of mobile phase compositions are studied. The conformity between theoretical calculated and experimental obtained RM-values of chromatographed substances for ...