The correlation of serum nesfatin-1 level in individuals with different glucose tolerance

Abstract

Objective To investigate changes of serum nesfatin level in individuals with different glucose tolerance,and its relationship with body mass index (BMI),plasma glucose,insulin sensitivity,etc.Methods 115 subjects were divided into three groups:normal glucose tolerance group (NGT group,n =33),impaired glucose tolerance group(IGT group,n =30)and newly diagnosed type 2 diabetes group(T2DM group,n=52).According to the BMI,T2DM group was divided into T2DM with obesity group (T2DM-OB group,n =22) and T2DM with normal body weight group(T2DM-NW group,n =30).The serum nesfatin-1level were measured by ELISA.Fasting plasma glucose (FPG),2 h postprandial blood glucose (2 h PG),in-terleukin-6 (IL-6),insulin,hemoglobin A1 c (HbA1 c),total cholesterol (TC),triglyceride (TG),high density lipoprotein-cholesterol(HDL-C),low density lipoprotein-cholesterol (LDL-C) were also measured.T2DM-OB group were given pioglitazone.After only BMI was higher than that in T2DM-NW group(P ＜ 0.05),then nes-fatin-1 level was measured.The homeostasis model assessment-insulin resistance (HOMA-IR),BMI were calculated as well.Results (1)The serum nesfatin-1 levels in T2DM group and IGT group were significant-ly higher than that in NGT group(P ＜ 0.05) [(1 780 ± 660) ng/L,(1 620 ± 590) ng/L vs.(1 390 ± 610)ng/L,P ＜0.05].(2)The serum nesfatin-1 level in T2DM-OB group was significantly higher than that in T2DM-NW group [(1897 ± 670)ng/L vs.(1 690 ± 650)ng/L,P ＜ 0.05].After treated with pioglitazone,the serum nesfatin-1 level in T2DM-OB group was significantly higher than that in T2DM-NW group[(1 791 ±634) ng/L vs.(1 690 ± 650) ng/L,P ＜ 0.05].(3) Nesfatin-1 level was positively correlated with TG,FPG,2 h PG,HbA1 c,IL-6,HOMA-IR,BMI (r =0.582,0.568,0.587,0.552,0.546,0.523,0.562).Multiple stepwise linear regression analysis showed that HOMA-IR,FPG and BMI were independently associated with serum nesfatin-1 level.Conclusion With the occurrence and aggravation of IGT and obesity,nesfatin-1 level increased,and nesfatin-1 may play a protective role in obesity and IGT. Key words: Nesfatin-1; Impaired glucose tolerance; Obesity