The correlation between retinopathy of prematurity susceptibility and +405G/C and 936 T/C polymorphism of vascular endothelial growth factor-A gene

Abstract

ObjectiveTo investigate the correlation between retinopathy of prematurity(ROP)susceptibility and +405G/C and 936T/C polymorphism of vascular endothelial growth factor A(VEGF-A)gene. Methods 99 ROP infants(ROP group)and 80 premature infants(control group)were enrolled in this study. There was no difference of gestational age, birth weight and preoxygenation time between the ROP and control group (P＞0. 05). The peripheral blood was collected, polymorphism genotypes and frequency of VEGF-A+405 and VEGF-A936 were measured by pyrosequencing. Results There are CC, GG, CG genotypes in VEGF-A+405 site, while CC, CT genotypes in VEGF-A 936 site. The VEGF-A+405 gene allele of C, G were 92,106 with the frequencies of 46.5％, 53.5％ in the ROP group, and 90, 70 with the frequencies of 56.2％, 43.8％ in the control group; the difference between two groups was not statistically significant (X2 =3. 396, P=0. 066). There was no correlation between VEGF-A+ 405 polymorphism and ROP susceptibility (OR=0. 675, OR95％ CI=0. 444, 1. 026). The VEGF-A 936 gene allele of C, G were 32,166 with the frequencies of 16.2％, 83.8％ in the ROP group, and 16, 144 with the frequencies of 10.0％, 90.0％ in the control group; the difference between two groups was not statistically significant (X2=2.894, P=0.089). There was no correlation between VEGF-A 936 polymorphism and ROP susceptibility (OR=0. 768, OR95％ CI=0. 711, 0. 829). ConclusionThere is no correlation between VEGF-A+405 or VEGF-A 936 polymorphism and ROP susceptibility. Key words: Retinopathy of prematurity/ genetics; Genetic predisposition to disease; Vascular endothelial growth factor A; Polymorphism, single nucleotide