The correlation between human leukocyte antigens-A, B, DRB1 high-resolution alleles and chronic renal failure caused by immunoglobulin-a nephropathy

Abstract

Objective To investigate the correlation between human leukocyte antigens-A, -B, -DRB1 (HLA-A, -B, -DRB1) high-resolution alleles and chronic renal failure (CRF) caused by immunoglobulin-a nephropathy (IgAN). Method The polymerase chain reaction-sequence-based typing (PCR-SBT) method was used to investigate the genotypes of HLA-A, -B and -DRB1 high-resolution alleles in 191 cases of CRF caused by IgAN (experimental group) and 503 healthy blood donors (control group). The alleles frequencies between two groups were compared and the association between CRF caused by IgAN and the polymorphism of HLA was analyzed. Result (1) There were 25 alleles at A locus, 48 alleles at B locus and 32 alleles at DRB1 locus in experimental group. (2) The genetic frequency of HLA-A*2901 [Pc=0.033, OR=10.738, 95% CI (1.193, 96.691)], HLA-DRB1*1106 [Pc=0.0001, OR=0.969, 95% CI (0.944, 0.994)], HLA-DRB1*1202[Pc=0.002, OR=1.859, 95% CI (1.259, 2.745)], HLA-DRB1*1401 [Pc=0.021, OR=0.984, 95% CI (0.967, 0.998)], HLA-DRB1*1602[Pc=0.015, OR=1.915, 95% CI (1.157, 3.17)] in experimental group was higher than in control group (P<0.05). Conclusion There is susceptibility association of HLA-A*2901, HLA-DRB1*1106, HLA-DRB1*1202, HLA-DRB1*1401, HLA-DRB1*1602 with CRF caused by IgAN. It is concluded that there is a close genetic and immunological correlation between HLA alleles and the pathogenesis of CRF caused by IgAN. Key words: Immunoglobulin-a nephropathy; Chronic renal failure; Human Leukocyte Antigens; Polymorphism; High-resolution; Poymerase chain reaction-sequence-based typing