Abstract
The article establishes the particularities of immune system dysfunction in the elderly during formation of a specific immune response to vaccination with a seasonal influenza vaccine depending on the level of seroconversion. The corrective effect of fucoidan — a sulfated polysaccharide extracted from brown algae Fucus evanescens — was shown in the formation of a specific immune response, accompanied by an increase in the expression of the activation molecules CD69 and CD86 on monocytes, a decrease in the expression density of CD20 on B-lymphocytes, and an increase in the relative content of memory cells (CD4 + CD45RO + -T-lymphocytes and cytotoxic CD8 + CD45RO + -T-lymphocytes). Fucoidan extracted from the brown algae Fucus evanescens of the Sea of Okhotsk can be used to increase the effectiveness of seasonal influenza vaccination in the elderly.
Highlights
The article establishes the particularities of immune system dysfunction in the elderly during formation of a specific immune response to vaccination with a seasonal influenza vaccine depending on the level of seroconversion
The corrective effect of fucoidan — a sulfated polysaccharide extracted from brown algae Fucus evanescens — was shown in the formation of a specific immune response, accompanied by an increase in the expression of the activation molecules CD69 and CD86 on monocytes, a decrease in the expression density of CD20 on B-lymphocytes, and an increase in the relative content of memory cells (CD4+CD45RO+-T-lymphocytes and cytotoxic CD8+CD45RO+-T-lymphocytes)
Fucoidan extracted from the brown algae Fucus evanescens of the Sea of Okhotsk can be used to increase the effectiveness of seasonal influenza vaccination in the elderly
Summary
Èññëåäîâàíèå ïðîâåäåíî â ýïèäåìè÷åñêèé ñåçîí ãðèïïà 2018–2019 ãã. â ñîîòâåòñòâèè ñ îñíîâíûìè ýòè÷åñêèìè ïðèíöèïàìè ýïèäåìèîëîãè÷åñêèõ èññëåäîâàíèé è Õåëüñèíêñêîé äåêëàðàöèè. Ïðîòîêîë èñïûòàíèÿ è âñå ñîîòâåòñòâóþùèå äîêóìåíòû áûëè îäîáðåíû Ýòè÷åñêèì êîìèòåòîì Ìåäèöèíñêîãî îáúåäèíåíèÿ ÄÂÎ ÐÀÍ. Ó÷àñòíèêè îïûòíîé ãðóïïû íà÷àëè ïðèíèìàòü ÁÀÄ Ôóêîëàì ýêñòðà, íà÷èíàÿ ñî äíÿ âàêöèíàöèè ïî 1 êàïñóëå 2 ðàçà â äåíü â òå÷åíèå 30 äíåé. Èììóíîôåíîòèïèðîâàíèå ëèìôîöèòîâ ïðîâîäèëè ìåòîäîì ïðîòî÷íîé öèòîìåòðèè (öèòîôëóîðèìåòð FACScan, BectonDickinson, ÑØÀ). Îöåíèâàëè àêòèâíîñòü êëåòî÷íîãî çâåíà âðîæäííîãî èììóíèòåòà (ìîíîöèòîâ) — ïî ýêñïðåññèè êîñòèìóëèðóþùèõ ìîëåêóë CD69, CD86, ñâèäåòåëüñòâóþùèõ îá àêòèâàöèè Ò-êëåòîê ïàìÿòè (CD4+CD45RO+ è CD8+CD45RO+) è ñóáïîïóëÿöèé Â-ëèìôîöèòîâ, çàâåðøàþùèõ äèôôåðåíöèðîâêó â ïëàçìàòè÷åñêèå êëåòêè (CD20+CD38++) ñ èñïîëüçîâàíèåì ìîíîêëîíàëüíûõ àíòèòåë: CD3, CD4, CD8, CD20, CD38, CD14, CD86, CD69, CD45RO (BekmanCoulter, ÑØÀ) è ñîîòâåòñòâóþùèõ èçîòîïè÷åñêèõ êîíòðîëåé. Ìîíîöèòû äèôôåðåíöèðîâàëè îò äðóãèõ êëåòîê ïî ïàðàìåòðàì FSC è SSC, à òàêæå ïî ýêñïðåññèè êëåòêàìè ìîëåêóë CD14. Âûáîðî÷íûå ïàðàìåòðû, ïðèâîäèìûå äàëåå â òàáëèöàõ, èìåþò ñëåäóþùèå îáîçíà÷åíèÿ: W — êðèòåðèé Shapiro–Wilk, îáúì àíàëèçèðóåìîé âûáîðêè (n), p — äîñòèãíóòûé óðîâåíü çíà÷èìîñòè.
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