Abstract
ObjectivesShen Fu Injection (SF), which consisted of Red ginseng extraction injection (RG) and prepared aconite extraction injection (RA), is a traditional Chinese medicine mainly used for various cardiac diseases. This study is to analyse SF's effects on cardiac performance and coronary circulation. And the coronary dilating effect and mechanism of the above three injections were also explored.MethodsMature male guinea pigs were used as our animal model. We employed two types of perfusion methods (constant pressure and constant flow) in vitro, using Langendorff heart preparations to observe the cardiac function and coronary response to SF (1/200). The coronary dilation effects of the above three injections (1/800, 1/400 and 1/200) were recorded at basal coronary resting tone and when coronary vessels were pre-contracted with a thromboxane A2 analogue (U46619), in the presence or the absence of the inhibitor of nitric oxide synthesis (L-NAME, 10−4 M), the blocker of Ca2+-activated potassium channel(TEA, 10−3 M), or the blocker of adenosine triphosphate (ATP)-sensitive potassium channel (glybenclamide) (10−5 M).ResultsWhen perfused with constant pressure, SF significantly increased coronary flow, left ventricular developed pressure (LVDP) and the rate-pressure product (RPP). When perfused with constant flow, SF produced a significant reduction in the coronary perfusion pressure (CPP), LVDP and RPP. The coronary vasodilatation response of the above three injections can be reduced by L-NAME but was unaffected by TEA or glybenclamide when coronary vessels were pre-contracted with U46619 but not at resting tone. SF, RG and RA can all up-regulate eNOS expression in the human umbilical vein cells (EA.hy926).ConclusionWe demonstrated that SF does not contribute to the inotropic change of myocardium whose improvement is due to alternation of coronary flow. The coronary dilation effect of SF was mediated through RG and RA, via promoting NO release.
Highlights
Shenfu Injection (SF) has been used in clinic as a medicine to treat cardiac disease including coronary heart disease, myocardial ischemia/reperfusion injury and especially these disease with heart insufficiency or congestive heart failure in China for a long time [1,2,3]
We reported that administration of Shenfu Injection, Red ginseng extraction injection (RG) and prepared aconite extraction injection (RA) all show coronary artery dilation effect, at least partially through Nitric Oxide (NO) release as they all up-regulate edothelium NO synthase (eNOS) level in endothelium cellsWe observed increases of the hemodynamics (i.e. left ventricular developed pressure (LVDP) and rate-pressure product (RPP)) induced by SF was the secondary responses to coronary vasodilation
When the hearts were perfused with constant pressure mode, administration of Shenfu injection
Summary
Shenfu Injection (SF) has been used in clinic as a medicine to treat cardiac disease including coronary heart disease, myocardial ischemia/reperfusion injury and especially these disease with heart insufficiency or congestive heart failure in China for a long time [1,2,3]. SF was consisted of Red Ginseng extraction and prepared aconite extraction [4]. Red Ginseng (Radix Ginseng Rubra) is the steamed root of Panax Ginseng C. A. Meyer, a perennial herb of the family Araliaceae that has been traditionally used (over 2,000 years) as a medicinal preparation in Republic of Korea, China, and Japan. The basis of the medicinal prowess remains unknown
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