Abstract
Numerous core particles measuring 45–50 nm in diameter were observed in the nuclei of cells infected with herpes simplex virus for 20 h. The sites for core-particle assembly appeared to be closely associated with viral DNA-replicating areas in the nucleus. The core particles were subsequently observed to be associated with the protein subunits that comprise the outer capsid of the virus. Outer capsids were formed only in areas in which core particles were present; however, numerous empty capsids (lacking a core particle) measuring 80–90 nm in diameter were also observed. The effect of trypsin digestion on thin sections of infected cells revealed that the core particles were selectively degraded by trypsin, but that the outer capsid and envelope remained relatively intact. The core particles appeared to be more sensitive to trypsin treatment after acquisition of a capsid and envelope.
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