The copepod Eurytemora affinis as a relevant species to assess estuarine sediment toxicity: Effects on gene expression and swimming behavior

Abstract

Compared to freshwater ecosystems, the health status of estuarine waters remains little studied despite their importance for many species. They represent a zone of interest for Human settlements that make them the final sink of pollution in both the water column and sediment. Once in sediments, pollutants could represent a threat to benthic as well as pelagic estuarine species through resuspension events. In the Seine estuary, the copepod Eurytemora affinis has been previously presented as a relevant species to assess resuspended sediment contamination through fitness-related effects at the individual level. The aim of the present study was to use E. affinis copepods to assess estuarine sediment-derived elutriates toxicity at environmental concentrations of particles using a molecular (i.e. transcriptomics) and a behavioral approach. Two sites along the Seine estuary were sampled. The analysis of sediments reveals that both sites have the same granulometric composition and close contamination profiles with the detection of PCBs, PAHs and pyrethroid insecticides. The transcriptomic analysis reveals that exposure to elutriates from both sites triggers the dysregulation of genes involved in biological function as defense response, immunity, ecdysone pathway or neurotoxicity with 66% and 36% of shared genes at the highest concentration for Tancarville and Fatouville. This analysis also reveals a higher count of dysregulated genes in the Fatouville site compared to the Tancarville (271 vs 148) despite their close contamination profile. These results emphasize the molecular approach sensitivity to assess environmental matrix toxicity with E. affinis. The analysis of the swimming behavior of E. affinis did not highlight significant effects after elutriate exposure. However, our strategy to assess E. affinis swimming behavior allows the discrimination of basal swimming behavior i.e. dark/light velocity changes and strong thigmotaxis behavior. Thus, it represents a promising standardized tool to assess copepods swimming behavior in ecotoxicological studies.