Abstract
Summary Several guinea pig precipitating immune sera, heated at 56°C for 30 min, no longer precipitated with homologous antigen. Heated sera were capable, however, of coprecipitation with homologous unheated serum and antigen, sensitizing guinea pigs to anaphylaxis, precipitation in liquid medium at pH 5, precipitation in agar gel at pH 5, 7 or 8.5, and hemagglutination. The presence of albumin globulin complexes in heated guinea pig immune serum could not be demonstrated. Guinea pig albumin was not detected immunologically after dissolving in excess antigen the precipitate formed by coprecipitation of heated antiserum with unheated antibody and homologous antigen.
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