The conversion of ovalbumin into plakalbumin, as followed in the pH-stat

Abstract

The limited proteolysis accompanying the transformation of ovalbumin into plakalbumin has been measured using the pH-stat. The reaction consists of at least two steps, the first step being the opening of a single peptide bond in a closed chain in the ovalbumin molecule, leading to the formation of an open intermediary protein form which has lost the ability of crystallizing in needles. In the further reaction this form is converted into plakalbumin.