Abstract
An α- d-galactosidase (EC 3.2.1.21), capable of converting group B into group O red cells, was isolated from the stem portion of taro. It was purified about 3000 fold by gel filtration and ion-exchange chromatography. The blood group-converting activity was demonstrated by hemolysis and hemagglutination studies. This activity is comparable to that of α- d-galactosidase isolated from coffee beans. Taro α- d-galactosidase also hydrolyzes (1→4)- and (1→6)-linked α- d-galactopyranosyl groups from d-galactose-containing glycoconjugates. Taro α- d-galactosidase has a low K m value (0.28mM), a low molecular weight (40 000), and a neutral optimal pH (6.0). At a final enzyme concentration of 30 units/mL in the incubation mixture, the conversion of group B into group O activity was completed within two hours, without apparent changes in the shape of the red cells.
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