The controlled porous glass (CPG) with reactive epoxy groups as support for affinity chromatography. II. Modified CPG as support of substrates or coenzyme of glucose oxidase (GOD) for its purification and immobilization

Abstract

AbstractControlled porosity glasses (CPG) are materials very widely employed as supports for chromatography and biotechnology. In this paper CPG with epoxy groups have been applied as carrier of FAD, glucose and 2‐deoxy‐D‐glucose. Glucose oxidase (GOD) was highly purified on these sorbents. The purified fraction of GOD was also immobilized on the activated CPG. The presented results suggest the higher stability of immobilized form of this enzyme in relation to the native enzyme.