Abstract
This study was implemented to figure out whether lncRNA HOTAIR/miR‐17‐5p/PTEN axis played a role that was opposite to Shenqifuzheng (SQFZ) injection in regulating the chemosensitivity of gastric cancer cells. The gastric cancer tissues were gathered and four gastric cancer cell lines were prepared, including BGC‐823, MGC‐803, SGC‐7901, and MKN28. Moreover, cisplatin, adriamycin, mitomycin, and 5‐fluoroura were managed as the chemo‐therapeutics, and SQFZ was prepared as a Chinese medicine. Striking distinctions of HOTAIR, miR‐17‐5p, and PTEN expressions were observed between gastric cancer tissues and para‐carcinoma normal tissues (P < 0.05). MKN28 was associated with the highest resistance to cisplatin, adriamycin, mitomycin, and 5‐fluoroura among all the cell types, and SQFZ significantly improved the MKN28 cells’ sensitivity to the drugs (P < 0.05). The over‐expressed HOTAIR and miR‐17‐5p, as well as under‐expressed PTEN tended to significantly facilitate the viability, EMT process and proliferation of MKN28 cells that were subject to treatment of chemo‐therapies (P < 0.05). SQFZ could amplify the effects of si‐HOTAIR, miR‐17‐5p inhibitor, and pcDNA‐PTEN on boosting the chemosensitivity of gastric cancer cells (P < 0.05). In addition, HOTAIR was also found to directly target miR‐17‐5p, and PTEN appeared to be subject to the modification of HOTAIR and miR‐17‐5p in its acting on the viability, proliferation, EMT process, and apoptosis of gastric cancer cells. The HOTAIR/miR‐17‐5p/PTEN axis could be regarded as the potential treatment targets for gastric cancer, and adjuvant therapy of SQFZ injection could assist in further improving the treatment efficacy of chemo‐therapies for gastric cancer.
Highlights
Gastric cancer is ranked second among all the malignancies considering its global morbidity and mortality, and its recognized clinicalJianguang Jia and Dankai Zhan contributed .characteristics are commonly comprised of insidious onset, missed diagnosis, and high recurrence rate.[1]
HOTAIR, miR‐17‐5p and PTEN have been, respectively, indicated to participate in development and chemosensitivity of gastric cancer, hardly any investigations have been focused on their synthetic contributions
All cases were reviewed by the pathologist, and were confirmed as gastric cancer based on histopathological assessment
Summary
Gastric cancer is ranked second among all the malignancies considering its global morbidity and mortality, and its recognized clinical. LncRNAs were extensively involved with cell differentiation, cellular metabolism and cell proliferation, which enabled their close correlation with onset and progression of diversified diseases.[6] Their expressions could biologically mark the process of tumor formation and development.[7]. HOTAIR, miR‐17‐5p and PTEN have been, respectively, indicated to participate in development and chemosensitivity of gastric cancer, hardly any investigations have been focused on their synthetic contributions. HOTAIR/miR‐17‐5p/PTEN axis, and SQFZ injection were, respectively, hypothesized as crucial participants in the oncogenesis and chemosensitivity of gastric cancer. They have not been investigated together within one study, this investigation was aimed to remedy this gap
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