Abstract
The article considers results of study of total concentration of nitrites and nitrates (NOx) in blood plasma of healthy individuals residing in St. Petersburg. The samples from 58 healthy individuals were analyzed and out of them - 31 aged 18-25 years and 27 aged 48-63 years. The concentration of NOx in blood plasma was determined using Griess reagent after reduction of nitrates under effect of NADH-dependent recombinant nitrate reductase from Arabidopsis thaliana. The surplus of NADH preventing Griess reaction was removed at the expense of of enzyme reaction in presence of lactate dehydrogenase and sodium pyruvate. The analysis and its calibration was carried out using additions of solution of sodium nitrate to samples of blood plasma without deproteinization. The data was obtained concerning variation of content of NOx in healthy individuals of various age. In particular, it is demonstrated that the level of total content of nitrogen oxide in the group of elder age was reliably higher (p=0.0001) as compared with donors aged 18-25 years.
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