The Contamination Degree of Characteristic Fungi in Jet Fuel Detected by ATP Bioluminescence

Zezhen Li,Yun Xiong,Bo Chen,Zhenqiao Ma

doi:10.3844/ajbbsp.2018.162.167

Abstract

Adenosine Triphosphate (ATP) lysate of the characteristic fungi in jet fuel was screened and three fluorescein-luciferase systems were compared in the paper. The correlation between ATP bioluminescence and traditional plate count for measuring the number of microorganism was also investigated and then a method of detecting the contamination degree of the characteristic fungi of jet fuel by ATP bioluminescence were initially established. The results show that the effect of ATP extraction taking surfactant Benzyldimethylhexadecylammonium chloride (BAC) as the microbial lysate was optimal and the optimal concentration and the action time were 0.15% and 30s respectively; The fluorescein-luciferase system after screening has a good detection limit, up to 10-15mol ATP; using ATP bioluminescence and traditional plate method to measure the microbial quantity, they have good correlation. Utilizing ATP bioluminescence to detect the contamination degree of the characteristic fungi in jet fuel can shorten the test time to 10min, which is suitable for rapid detection and has good application prospects.

Highlights

Since 1930s, there are many studies on the microbial contamination of jet fuel (Graef, 2003)
The one-dimensional linear regression equation obtained is Y = 0.8080X+8.3884, R2 = 0.9889; in the range of 10-8~3×10-11 mol/L, fluorescence intensity has a good linear relationship with Adenosine Triphosphate (ATP) concentration that bioluminescence can be used to measure the degree of microbial contamination in jet fuel
The reaction time of lysate is shown in Fig. 2B; it can be seen that the fluorescence intensity is reduced rapidly with time growth, which may be determined by the reaction process of fluorescence system and ATP

Summary

Introduction

Since 1930s, there are many studies on the microbial contamination of jet fuel (Graef, 2003). The number of viable cells can be calculated by determining the ATP concentration in the sample. The luminescence intensity is proportional to the ATP concentration. The ATP concentration can be quantified by measuring luminescence intensity of the luminescent system. The ATP bioluminescence detection procedure generally includes: Sampling, ATP extraction, addition of fluorescein and luciferase, measurement of bioluminescence, determination of ATP concentration and viable cell count. ATP cannot be measured without treatment of the sample. The luciferase bioluminescence agent was used to measure the bioluminescence of ATP by a luminescence detector. People’s researches on the microorganisms in jet fuel are mainly focusing on the identification and control measures of pollution strains (Andreolli et al, 2016).

Methods

Results

Discussion

Conclusion