Abstract
BackgroundPorcine epidemic diarrhea (PED) is a contagious intestinal disease caused by porcine epidemic diarrhea virus (PEDV) characterized by vomiting, diarrhea, anorexia, and dehydration, which have caused huge economic losses around the world. At present, vaccine immunity is still the most effective method to control the spread of PED. In this study, we have constructed a novel recombinant L. casei-OMP16-PEDVS strain expressing PEDVS protein of PEDV and OMP16 protein of Brucella abortus strain. To know the immunogenicity of the recombinant L. casei-OMP16-PEDVS candidate vaccine, it was compared with BL21-OMP16-PEDVS-F, BL21-OMP16-PEDVS, and BL21-PEDVS recombinant protein.ResultsThe results showed that we could detect higher levels of IgG, neutralizing antibody, IL-4, IL-10, and INF-γ in serum and IgA in feces of L. casei-OMP16-PEDVS immunized mice, which indicated that L. casei-OMP16-PEDVS candidate vaccine could induce higher levels of humoral immunity, cellular immunity, and mucosal immunity.ConclusionTherefore, L. casei-OMP16-PEDVS is a promising candidate vaccine for prophylaxis of PEDV infection.
Highlights
Porcine epidemic diarrhea (PED) is caused by porcine epidemic diarrhea virus (PEDV) with symptoms including diarrhea, vomiting, anorexia, dehydration, and weight loss in piglets [1, 2]
These results indicated the successful construction of pET28a-PEDVS, pET28aOMP16-PEDVS, and pVE5523-OMP16-PEDVS recombinant plasmids
The pVE5523-OMP16-PEDVS protein was verified in the cultural supernatant of L. casei-pVE5523OMP16-PEDVS strain
Summary
Porcine epidemic diarrhea (PED) is caused by porcine epidemic diarrhea virus (PEDV) with symptoms including diarrhea, vomiting, anorexia, dehydration, and weight loss in piglets [1, 2]. To control the spread of PEDV, most kinds of vaccines are constructed, such as aluminumhydroxide-adjuvanted inactivated vaccine, bivalent inactivated Transmissible Gastroenteritis Virus (TGEV) and PEDV vaccine, and attenuated PEDV vaccine [4]. These vaccines play an important role in controlling PED, they all have their defects. Porcine epidemic diarrhea (PED) is a contagious intestinal disease caused by porcine epidemic diarrhea virus (PEDV) characterized by vomiting, diarrhea, anorexia, and dehydration, which have caused huge economic losses around the world. Results: The results showed that we could detect higher levels of IgG, neutralizing antibody, IL-4, IL-10, and INF-γ in serum and IgA in feces of L. casei-OMP16-PEDVS immunized mice, which indicated that L. casei-OMP16-PEDVS candidate vaccine could induce higher levels of humoral immunity, cellular immunity, and mucosal immunity
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