The construct of triple responsive nanocomposite and its antibacterial effect

Abstract

The current serious mismatch between the increasing severity of bacterial infections and antibiotic production capacity urgently requires the emergence of novel antimicrobial materials. In this paper, dopamine methacrylamide (DMA) and N-isopropylacrylamide (NIPAM) were polymerized as the monomers into a block copolymer poly(dopamine methacrylamide-block-N-isopropylacrylamide) (P(DA-NIP)) and then encapsulated with polydopamine-coated magnetic nanoparticle clusters (MNC) to produce an antibacterial nanocomposite (MNC@P(DA-NIP)). This nanocomposite has triple responses respectively to light, heat and magnetism, which endow MNC@P(DA-NIP) with the abilities to kill bacteria effectively and capture/release bacteria conveniently. Under near-infrared (NIR) light irradiation, MNC@P(DA-NIP) could significantly elevate the temperature through photothermal conversion. The increased temperature favored both the capture of bacteria on MNC@P(DA-NIP), and the damage of bacterial cells, causing bacterial death almost completely. While low temperatures could promote the release of dead bacteria from the nanocomposites, might through the recovery of the hydrophilic state of the outlayer PNIPAM. Moreover, thanks to the magnetic responsibility, MNC@P(DA-NIP) could be easily separated from the bacterial cells and perform better biofilm penetration. The results showed that the antibacterial effect of MNC@P(DA-NIP) was 3.5 times higher than that of MNC, and the recycling capacity of MNC@P(DA-NIP) was better than MNC@PDA. What’s more, MNC@P(DA-NIP) possessed the excellent anti-biofilm properties under magnetic field (MF) and NIR. The most important features of the triple-responsive nanocomposites are excellent antibacterial effect, good recyclability and easy preparation, which provide the nanocomposites with great potential in eliminating harmful bacterial cells.