Abstract
Ethnopharmacological relevance Anisomeles indica (L.) Kuntze. (Labiatae), popularly known as ‘yu-chen-tsao’, has been traditionally used as anti-inflammatory agent. Aim of the study Investigate the chemical constituents from the whole plants of Anisomeles indica, and evaluate their in vitro anti-inflammatory activities. Results The combined MeOH extract was successively partitioned with CHCl 3 and n-butanol, then submitted to several column chromatographic, and HPLC purification procedures which led to the isolation of one cembrane-type diterpenoid ( 3), two benzenoids ( 4 and 5), five flavonoids ( 1, 2, 6, 7 and 14), and six phenyl propanoids ( 8– 13). The compounds 1– 14 were examined for their inhibitory effects on inflammatory mediator's enhanced production from LPS/IFN-γ-stimulated macrophages. Among these, ovatodiolide ( 3) exhibited potent inhibition on NO, TNF-α and IL-12 enhanced production at a concentration of 5 μM, followed by pedalitin ( 1), scutellarein 7- O-β- d-glucuronide methyl ester ( 6), and acteoside ( 12) at 40 μM ( P < 0.05). Furthermore, 2 μM of 3, and 20 μM of 1 and 6 significantly ( P < 0.05) arrested the cell cycle of Con A-stimulated spleen cells at the G0/G1 stage. Conclusion This is the first report on the presence of compounds 1 and 4– 13 in this plant and of the potent anti-inflammatory activity of 1, 3, 6 and 12 in vitro. These compounds may account for the use of Anisomeles indica in folk medicine to treat inflammation.
Published Version
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