The Consequences of Mixed-Species Malaria Parasite Co-Infections in Mice and Mosquitoes for Disease Severity, Parasite Fitness, and Transmission Success.

Jianxia Tang,Richard Culleton,Jun Cao,Thomas J Templeton

doi:10.3389/fimmu.2019.03072

Jianxia Tang, Richard Culleton + Show 2 more

Open Access

https://doi.org/10.3389/fimmu.2019.03072

Copy DOI

Abstract

The distributions of human malaria parasite species overlap in most malarious regions of the world, and co-infections involving two or more malaria parasite species are common. Little is known about the consequences of interactions between species during co-infection for disease severity and parasite transmission success. Anti-malarial interventions can have disproportionate effects on malaria parasite species and may locally differentially reduce the number of species in circulation. Thus, it is important to have a clearer understanding of how the interactions between species affect disease and transmission dynamics. Controlled competition experiments using human malaria parasites are impossible, and thus we assessed the consequences of mixed-species infections on parasite fitness, disease severity, and transmission success using the rodent malaria parasite species Plasmodium chabaudi, Plasmodium yoelii, and Plasmodium vinckei. We compared the fitness of individual species within single species and co-infections in mice. We also assessed the disease severity of single vs. mixed infections in mice by measuring mortality rates, anemia, and weight loss. Finally, we compared the transmission success of parasites in single or mixed species infections by quantifying oocyst development in Anopheles stephensi mosquitoes. We found that co-infections of P. yoelii with either P. vinckei or P. chabaudi led to a dramatic increase in infection virulence, with 100% mortality observed in mixed species infections, compared to no mortality for P. yoelii and P. vinckei single infections, and 40% mortality for P. chabaudi single infections. The increased mortality in the mixed infections was associated with an inability to clear parasitaemia, with the non-P. yoelii parasite species persisting at higher parasite densities than in single infections. P. yoelii growth was suppressed in all mixed infections compared to single infections. Transmissibility of P. vinckei and P. chabaudi to mosquitoes was also reduced in the presence of P. yoelii in co-infections compared to single infections. The increased virulence of co-infections containing P. yoelii (reticulocyte restricted) and P. chabaudi or P. vinckei (predominantly normocyte restricted) may be due to parasite cell tropism and/or immune modulation of the host. We explain the reduction in transmission success of species in co-infections in terms of inter-species gamete incompatibility.

Highlights

Eight malaria parasite species are infectious to humans; namely, Plasmodium falciparum, Plasmodium vivax, Plasmodium malariae, Plasmodium ovale wallikeri, Plasmodium ovale curtisii, Plasmodium knowlesi, Plasmodium cynomolgi, and Plasmodium simium
Mixed species infections resulted in higher parasitaemia than either of their constituent species in single infections (Figure 1D) and peak parasitaemia occurred on the same day PI as the more virulent of the constituent species; except for P. y. yoelii clone CU (PyCU) + P. v. lentum clone DS (PvDS) in which peak parasitaemia occurred between days 8 and 11, compared to the PvDS single
Our results indicate that the interactions between malaria parasites co-infecting the same host can have dramatic consequences for the severity of the disease they cause

Summary

Introduction

Eight malaria parasite species are infectious to humans; namely, Plasmodium falciparum, Plasmodium vivax, Plasmodium malariae, Plasmodium ovale wallikeri, Plasmodium ovale curtisii, Plasmodium knowlesi, Plasmodium cynomolgi, and Plasmodium simium The latter three species are parasites of non-human primates, and cause zoonotic malaria in humans [1,2,3]. Mixed species infections of human malaria parasites are well-documented in natural [8,9,10,11,12] and experimental [e.g., [13, 14]] settings They are studied regarding diagnosis [15,16,17], treatment [18], immune response [19], virulence [12, 20], transmission [21,22,23], and in discussions of public health policy [6]. The virulence of malaria infection is of interest in the context of co-infection with other pathogens, such as HIV and Schistosoma [24,25,26]

Methods

Results

Conclusion