The Conditions under Which Consolidation of Serial-Order Conditioned Fear Requires De Novo Protein Synthesis in the Basolateral Amygdala Complex.

Abstract

Consolidation of conditioned fear to a stimulus (S1) paired with shock requires de novo protein synthesis in the basolateral amygdala complex (BLA), whereas consolidation of conditioned fear to a stimulus (S2) paired with the fear-eliciting S1 requires DNA methylation but not de novo protein synthesis in the BLA. The present experiments merged these protocols by exposing rats to pairings of a serial S2-S1 compound and shock to examine if/when protein synthesis in the BLA is required to consolidate fear to S2. Rats received a BLA infusion of the protein synthesis inhibitor, cycloheximide, immediately after the S2-S1-shock session and were subsequently tested with S2. The infusion disrupted consolidation of fear to S2 when there had been no prior training of S1 (Experiment 1), the prior training had consisted of unpaired presentations of S1 and shock (Experiment 4), or in pairings of S1 and sucrose (Experiment 5). Consolidation of fear to S2 was unaffected by the infusion of cycloheximide but was disrupted by the DNA methyltransferase inhibitor, 5-AZA, when S1 had been previously fear-conditioned (Experiments 2a, 2b, and 3). These findings imply that what has already been learned about S1 determines the BLA processes that consolidate fear to S2. The already-fear-conditioned S1 blocks the S2-shock association that otherwise forms (and whose consolidation requires de novo protein synthesis in the BLA) while simultaneously acting as a learned source of danger for its S2 associate (whose consolidation requires DNA methylation but not de novo protein synthesis in the BLA).SIGNIFICANCE STATEMENT Protein synthesis is widely thought to be crucial for consolidating new learning into stable memories, including the consolidation of conditioned fear memories in the basolateral amygdala complex (BLA). However, our data provide clear evidence that the requirement for protein synthesis to consolidate conditioned fear in the BLA depends on an animal's previous training history, and the type of learning that is consolidated. Further, within the BLA, our data show that DNA methylation, and not protein synthesis, is necessary to consolidate higher-order conditioned fear, indicating that epigenetic mechanisms may provide a more fundamental mnemonic substrate.