The condition of the intracellular metabolism of peripheral blood leukocytes in arterial hypertension (experimental study)

Abstract

Objective: the comparative analysis of the features of intracellular metabolism of polymorphonuclear leukocytes (PNL) in the peripheral blood of rats with the normal blood pressure and hereditary stress-induced arterial hypertension (ISIAH). Material and methods. Indicators of carbohydrate and lipid metabolism, activity of some key redox enzymes in leukocytes of rats as well as a level of PNL functional activity were examined by histochemical methods. Results. The study determined that the stable increased blood pressure is accompanied by hypertrophy of the left ventricle of the heart, degenerative changes in cardiomyocytes and the decreased microcirculatory bed density. In this case amount of intracellular glycogen in leukocytes was decreased in 23% at unchanged amount of intracellular lipids. Activities of intracellular ATP-ase and myeloperoxidase were reduced in 22% with non-significant increasing of succinate dehydrogenase activity (in 13%). Amount of PNL with positive NBT-test was increased in 35% in the rats of ISIAH. Conclusions. Analysis of the studied metabolic indicators of PNL shows disturbance of the energy-supplying and enzymatic processes in the leukocytes on the background of arterial hypertension.