Abstract
Interstitial fluid was sampled from rats by subcutaneously implanted diffusion chambers and analyzed for calcium, inorganic phosphate and protein. The ultrafiltrable fractions of calcium and inorganic phosphate in the interstitial fluid were estimated by a dialyzing chamber implanted in the same way. The concentration of total calcium in the interstitial fluid was found to be 8.80 mg/100 ml, or 12% lower than in serum from the same animals. The concentration of inorganic phosphate was found to be 16% lower than serum-P. The ultrafiltrable fractions of calcium and inorganic phosphate in the interstitial fluid were found to be 71% and 92% of total Ca and P respectively. The concentration of protein in the interstitial fluid was about 62% of the serum protein value, and thus considerably higher than the commonly accepted values. The disc electrophoretic pattern of the interstitial fluid protein showed only minor quantitative differences from that of serum.
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