Abstract
The precipitation of poliovirus by nucleic acid at low pH represents a new, highly satisfactory method for the concentration of virus from large volumes of tissue culture filtrate. This method has been used as a first step in a procedure for the purification of poliovirus. Virtually pure preparations so obtained have permitted the redetermination of the extinction coefficient of poliovirus ( E 260 190 = 81.6) and the determination of the specific refractive increment of the virus ( Δ n = 0.00174). Sufficient quantities of the virus became available through the use of this procedure for the determination of the elementary composition of the virus. Purified preparations of poliovirus obtained by the use of this method were inactivated with formaldehyde and were found to induce neutralizing antibodies in animals and man.
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