The concentration and duration of the circulation of ampicillin in the body of chickens with aerosol use

Abstract

It has been established that antibiotics that reach a high concentration in tissues and blood serum are highly effective, but even at a relatively low concentration, some classes of antimicrobial drugs have a sufficient bactericidal effect, which deserves some attention. In this regard, it was necessary to establish the timing of the removal of ampicillin from the body of the bird during its aerosol use, which makes it possible to determine the time of active action of the drug and assess the possible effectiveness of this treatment regimen in various respiratory diseases.The material on the study of the distribution of ampicillin in the organs and tissues of 25-30-day-old chickens of egg and meat breeds with its double-edged aerosol administration at the rate of 250 mg per meter cubic inhalation is presented. The material for the study of the pharmacokinetics of the drug was: blood serum, liver, lungs, muscular stomach, skeletal muscle, kidneys. From the organ under study, a hinge (on analytical balances) of 1 gram was taken. Next, a buffer was poured at the rate of 1 g of attachment + 3 ml of buffer. Crushed to a homogeneous state and centrifuged for 15 min at 2000 rpm. The supernatant was used for research. To quantify ampicillin, the microbiological method of diffusion in agar was used. The activity of the tested antimicrobial agent was determined by comparing the zones of inhibition of the growth of microorganisms caused by the known concentrations of the investigational drug and the officially registered state standard sample of the antibiotic. The diameters of the growth retardation zones of microorganisms were determined using a compass and a ruler. Then, according to the derived calibration curve, the content of ampicillin in the organs and tissues of the body of chickens was stated.