Abstract
THE COMPOSITION OF UTERINE AND ENDOMETRIAL NUCLEIC ACID
Highlights
It appears that certain steroid hormones, in the process of eliciting marked physiological responses by alteration in growth, cell type, and function in such a “target organ” as the uterus, alter the composition of the nucleic acids prepared from this organ
The tissues were chosen with the purpose of evaluating representative morphological phases of endometrial growth
Methods used for the preparation, hydrolysis, chromatography, and estimation of the purines and pyrimidines are discussed
Summary
Tissue-Fresh human endometrial samples were obtained and classified as described previously [10]. Preparation of DNA-The tissue residue remaining after PNA extraction was washed with absolute alcohol and ether and dried. Of 1 N HCl at 100” for 1 hour in sealed tubes, as described by Smith and Markham [15]. This serves to hydrolyze the PNA to guanine, adenine, cytidylic acid, and uridylic acid. Chromatography separates guanine, adenine, cytosine, uracil, and thymine in that ascending order. 0.4 21.1 f 0.4 21.2 f 0.3 21.0 adenine, uracil, and uridylic acid, 275 rnp for cytosine, 280 rnp for cytidylic acid, and 265 rnp for thymine in the Beckman spectrophotometer in the manner described previously [10] 20.6 f. 0.4 21.1 f 0.4 21.2 f 0.3 21.0 adenine, uracil, and uridylic acid, 275 rnp for cytosine, 280 rnp for cytidylic acid, and 265 rnp for thymine in the Beckman spectrophotometer in the manner described previously [10]
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