Abstract

The production of ethanol in Brazil is based on sugarcane juice, however other biomasses can be used for this process, such as sweet sorghum. However, some nutrients can interfere with fermentation, such as the presence of metals, carbon and nitrogen sources, which can affect the fermentation capacity of yeasts. Thus, this study aims to analyze the presence of fundamental nutrients present in saccharine substrates, as well as their assimilation and conversion of ethanol by the yeast Pedra-2. Samples of sugarcane and sorghum juice were obtained, in which analysis of the presence of metals was carried out using acid digestion and the levels determined by atomic flame absorption spectroscopy. The amino acid analysis was performed on the saccharine substrates at a concentration of 22 ºBrix, before and after fermentation, and analyzed by high-performance liquid chromatography and the concentration of ethanol by gas chromatography. The sorghum broth showed higher amounts of available amino acid metals. The yeast Pedra-2 showed better fermentative performance in the sorghum broth. We can conclude that the sorghum broth represents an important substrate to be used to increase the sustainability and production of ethanol in Brazil.

Highlights

  • Renewable biomasses emerge on the world stage as an alternative to non-renewable natural resources, especially concerning their use as a raw material for obtaining energy, with a view to the demand for this resource, which is related to society's consumption modes modern (Azevedo & Azevedo Lima, 2016)

  • The calcium that acts in cell protection in the presence of high levels of ethanol, as it acts as an intracellular signal against homeostasis (Couchesne et al, 2011)

  • The sorghum juice showed higher amounts of metals in relation to the sugarcane juice, as well as a greater amount of available amino acids and the most assimilated by yeast, were: were tryptophan, cysteine, proline, arginine, alanine, threonine and isoleucine, as a result of this, this substrate has a great potential to be used in the production of ethanol

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Summary

Introduction

Renewable biomasses emerge on the world stage as an alternative to non-renewable natural resources, especially concerning their use as a raw material for obtaining energy, with a view to the demand for this resource, which is related to society's consumption modes modern (Azevedo & Azevedo Lima, 2016). Countries that can produce large quantities of natural resources with energy potential are in promising conditions (Ferreira, Silva & Ferreira, 2013). The growing interest in biomass transformation processes and the possibilities of application in different industrial processes have been demanding the development of technologies that provide a more efficient energy conversion from biomass. In this context, Brazil has numerous advantages compared to other countries, as it has a large territorial extension, with fertile soils, water availability and tropical climate, favourable conditions for the production of a variety of plant varieties with bioenergetic potential. Sugarcane (Saccharum officinarum), is a successful example of biomass with

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