The composition and stability of the vaginal microbiota of normal pregnant women is different from that of non-pregnant women

Roberto Romero,Adi L Tarca,Jacques Ravel,Tinnakorn Chaiworapongsa,Piya Chaemsaithong,Sonia S Hassan,Ronald F Lamont,Douglas W Fadrosh,Marisa Galuppi,Pawel Gajer,Lorraine Nikita,Jezid Miranda

doi:10.1186/2049-2618-2-4

Abstract

BackgroundThis study was undertaken to characterize the vaginal microbiota throughout normal human pregnancy using sequence-based techniques. We compared the vaginal microbial composition of non-pregnant patients with a group of pregnant women who delivered at term.ResultsA retrospective case–control longitudinal study was designed and included non-pregnant women (n = 32) and pregnant women who delivered at term (38 to 42 weeks) without complications (n = 22). Serial samples of vaginal fluid were collected from both non-pregnant and pregnant patients. A 16S rRNA gene sequence-based survey was conducted using pyrosequencing to characterize the structure and stability of the vaginal microbiota. Linear mixed effects models and generalized estimating equations were used to identify the phylotypes whose relative abundance was different between the two study groups. The vaginal microbiota of normal pregnant women was different from that of non-pregnant women (higher abundance of Lactobacillus vaginalis, L. crispatus, L. gasseri and L. jensenii and lower abundance of 22 other phylotypes in pregnant women). Bacterial community state type (CST) IV-B or CST IV-A characterized by high relative abundance of species of genus Atopobium as well as the presence of Prevotella, Sneathia, Gardnerella, Ruminococcaceae, Parvimonas, Mobiluncus and other taxa previously shown to be associated with bacterial vaginosis were less frequent in normal pregnancy. The stability of the vaginal microbiota of pregnant women was higher than that of non-pregnant women; however, during normal pregnancy, bacterial communities shift almost exclusively from one CST dominated by Lactobacillus spp. to another CST dominated by Lactobacillus spp.ConclusionWe report the first longitudinal study of the vaginal microbiota in normal pregnancy. Differences in the composition and stability of the microbial community between pregnant and non-pregnant women were observed. Lactobacillus spp. were the predominant members of the microbial community in normal pregnancy. These results can serve as the basis to study the relationship between the vaginal microbiome and adverse pregnancy outcomes.

Highlights

This study was undertaken to characterize the vaginal microbiota throughout normal human pregnancy using sequence-based techniques
community state type (CST) IV-A was characterized by a roughly equal number of Peptoniphilus, Anaerococcus, Corynebacterium, Finegoldia, Prevotella and a few other taxa. Those of CST IV-B had higher relative abundance of the genus Atopobium and were characterized by the presence of L. iners, Prevotella, Sneathia, Gardnerella, Ruminococcaceae, Parvimonas, Mobiluncus and other taxa previously shown to be associated with bacterial vaginosis [96]
To characterize the nature of the changes during pregnancy, we evaluated the ability of a community to shift to CST IV (A or B) by computing the Jensen-Shannon distance between each community state and the mean community state of all samples assigned to CST IV-A and CST IV-B

Summary

Introduction

This study was undertaken to characterize the vaginal microbiota throughout normal human pregnancy using sequence-based techniques. We compared the vaginal microbial composition of non-pregnant patients with a group of pregnant women who delivered at term. The presence of Lactobacillus spp. is associated with a healthy state and is thought to protect reproductive age women from non-indigenous pathogens [5-26], certainly by contributing to the maintenance of a low vaginal pH (

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